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EZ-QC™ mRNA Capping Efficiency Assay Kit
CELLSCRIPT™Available: Worldwide
Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis
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- The EZ-QC™ mRNA Capping Efficiency Assay Kit provides quantitative capping efficiency (percent capped RNA content) analysis of synthesized mRNA. 5'-cap structures can be added co-transcriptionally or post-transcriptionally to in vitro transcribed (IVT) RNA to produce Cap 0 and/or Cap 1 structures as a step in the mRNA synthesis process. Since only capped RNAs will be expressed in cells, it is important to have the highest possible percentage of capped RNAs present in a sample.
- The EZ-QC™ mRNA Capping Efficiency Assay Kit simplifies analysis and replaces tedious and expensive capping efficiency determination methods such as HPLC and mass spectrometry, instead allowing for determination based upon standard polyacrylamide gel electrophoresis (PAGE) methodology.
- The EZ-QC™ mRNA Capping Efficiency Assay utilizes a chimeric RNA:DNA:RNA Targeting Oligonucleotide (user provided) which hybridizes to the 5'-end of an RNA mixture. This hybridization complex serves as a substrate for RNase H cleavage releasing the capped and uncapped 5'-end fragments which can subsequently be resolved via PAGE and quantified by gel band analysis allowing for the calculation of the percent capped RNA content of the sample.
- The EZ-QC™ mRNA Capping Efficiency Assay does not differentiate between Cap 0 and Cap 1 capped RNAs, only between capped (Cap 0 + Cap 1) and uncapped RNAs.
