Flow Cytometry Services
Unlock immune insights with Crown Bioscience’s high-throughput flow cytometry—precisely characterize immune cell populations across tissues to elucidate drug mechanisms, assess pharmacodynamics, and optimize immunotherapies.
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Comprehensive Flow Cytometry Services for In-Depth Immunophenotyping
In the realm of oncology and immunology research, understanding the intricate dynamics of the immune system is paramount. Crown Bioscience offers advanced flow cytometry services designed to provide detailed immunophenotyping, enabling researchers to gain critical insights into drug mechanisms of action (MoA), pharmacodynamics (PD), and therapeutic efficacy.
Key Features and Capabilities:
- High-Throughput Analysis: Utilizing state-of-the-art equipment, including BD FACSCalibur™, BD Accuri™ C6, NovoCyte, Beckman Gallios, and BD LSRFortessa™ X-20 (capable of up to 17-color detection), Crown Bioscience ensures rapid and precise analysis of various cell types across multiple tissues such as tumors, blood, spleen, and lymph nodes.
- Comprehensive Immunophenotyping Panels: Standard and customizable panels are available for both murine and human samples. These panels can identify a wide range of immune cell populations, including T cells (CD4⁺, CD8⁺, regulatory T cells), B cells, natural killer (NK) cells, myeloid-derived suppressor cells (MDSCs), macrophages (M1 and M2), and more.
- Optimized Tissue Dissociation Protocols: With over 20 pre-optimized protocols for tumor tissue dissociation, Crown Bioscience ensures high-quality single-cell suspensions, preserving cell surface markers essential for accurate immunophenotyping.
- Stringent Quality Controls: To guarantee data integrity, stringent controls and manual compensation are employed during staining, processing, and gating procedures. Centralized data processing and analysis further minimize human error.
- Expert Consultation: A dedicated team of flow cytometry experts collaborates with researchers to determine optimal dissociation protocols, panel configurations, and gating strategies tailored to specific study requirements.
- Dynamic Reporting: Researchers receive comprehensive reports featuring raw data, statistical analyses, observations, actionable recommendations, and data visualizations. These reports are accessible through a secure cloud portal, facilitating seamless data review and collaboration.
Applications in Research:
- Mechanism of Action Studies: By analyzing changes in immune cell populations post-treatment, researchers can elucidate the MoA of investigational drugs.
- Pharmacodynamic Assessments: Monitoring immune responses over time aids in understanding the PD effects of therapeutics.
- Biomarker Discovery: Detailed immunophenotyping can uncover potential biomarkers predictive of treatment response or resistance.
- Therapeutic Efficacy Evaluation: Assessing the impact of therapies on specific immune cell subsets provides insights into their efficacy and potential side effects.
