GenSmart Codon Optimization tool

GenSmart™ Codon Optimization uses a newly-designed algorithm based on the "Population Immune Algorithm", which takes advantage of both population genetics and immunology theories. In this approach, more than 200 factors involved in gene expression, including GC content, codon usage and content index, RNase splicing sites, and cis-acting mRNA destabilizing motifs are screened and validated. Instead of applying a single-factor simulation to computing, a multifactor approach is employed to ensure that all key factors in a certain target gene sequence carry weight. As a result, each gene optimization is fully customized to maximize the chance of obtaining a functional and active protein.