GLP-1 ELISA

Glucagon-like peptide-1 (GLP-1) is a 30-amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after selective cleavage by dipeptidyl peptidase-4 (DPP-4). GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity.

Researchers have struggled with finding reliable GLP-1 assays. A 2014 study by Jens J Holst's group in Denmark evaluated several commercially available GLP-1 immunoassays and concluded that the specificity and sensitivity of the various assays vary significantly.

In consultation with several key opinion leaders, Ansh Labs has screened countless monoclonal antibodies and tested numerous assay validation protocols to identify the optimal antibody pair. We are confident that our GLP-1 ELISA kit will set the standard in GLP testing allowing researchers to accurately quantify GLP-1.

The Ansh Labs' Glucagon-like peptide-1 (GLP-1) enzyme linked immunosorbent assay (ELISA) kit, a quantitative two-step sandwich immunoassay, provides materials for the quantitative measurement of GLP-1 in EDTA plasma and other biological fluids. Same day results, simple two-step procedure with total 1.5 hour incubation at RT avoids the complex or overnight procedures inherent in current GLP-1 assays. Measures GLP-1 (9-36a) at 100%, GLP-1 (7-36a) at 44%, with no detectable cross-reactivity to Oxyntomodulin, Glucagon, GLP-2, or GRPP.

• Sensitive to 5.6 pg/mL (1.8 pmol/L)
• Analytical measurable range of 20-313 pg/mL (6.4-136 pmol/L) allows for accurate quantitations
• Sample size of 100 μL for human and 20 μL for rodents, no extraction needed, no special collection tube required

Ansh Labs, located just south of Houston Texas, is an FDA registered manufacturer of diagnostic test kits, antibodies, antigens, and proteins. Our scientists are experts in immunoassay development and have developed some of the most challenging hormone assays helping to advance research and clinical diagnostics in reproductive function, growth disorders, metabolism, and more.