Glucose Uptake-Glo™ Assay
A Simple, Non-Radioactive Assay to Measure Glucose Uptake Inside Cells
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Great replacement for radioactive 2-DG assay.
Measure glucose uptake in cells
Great alternative to radioactive 2-DG assays. However, not specific to 2-deoxyglucose and it will detect endogenous glucose in the cells, which could lead to high background. Several additional components are required that aren't obvious until you read the protocol in detail. Normalizing is difficult because of lysis buffer components. Was able to scale down to 384 well format. Functioned in principle, but potentially difficult to use at this scale without good lab practice and tools. Is my first choice for non-radioactive glucose uptake.
Review Date: 25 Oct 2019 | Promega Corp.
Easy to use kit.
Analyze glucose uptake in adipocytes
Simple to use kit without radioactivity. Luminescent plate reader required. Works well with adipocytes differentiated on 96-well plates.
Review Date: 25 Oct 2019 | Promega Corp.
Easy-to-use kit, but expensive and not as great results with L6 cells.
Glucose uptake in skeletal muscle cells (L6)
This kit is very straightforward and easy to use. The only down sides are the cost (it is quite expensive) and the fact that for the cells I use, I don't get as strong of an insulin-stimulated glucose uptake response as I'd like (despite the fact that I am following the kit's instructions for L6 cells).
Review Date: 25 Oct 2019 | Promega Corp.
Really easy and quick results.
Measure glucose uptake in cells
Bought after a suggestion from one of the Promega reps. Really easy to use and gives reproducible results. All of the protocol can be done in the day.
Review Date: 27 Jun 2019 | Promega Corp.
Glucose metabolism is a key process in many organisms. A lack of insulin-stimulated glucose uptake is associated with type 2 diabetes, while high glucose uptake is a sign of the high glycolytic rates associated with cancer. Measuring glucose uptake can determine the effects of various treatments for diabetes and cancer.
The gold standard method of assaying glucose uptake relies on detection of radio-labelled glucose analogs. Alternative colorimetric and fluorometric glucose uptake assays often lack the sensitivity and robustness needed to reliably measure glucose uptake in cells.
