HiSeq 2500 Sequencing System
Power and efficiency for large-scale genomics. The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. No other system lets you perform so many applications so quickly and easily. Choose between rapid run and high output mode…
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Recommend
Bioinformatics
The hiSeq system is novel and enables accurate analysis of RNASeq data (from post mortem human Alzheimer's Disease (AD)/Parkinson's Disease (PD) patients). The data can be computationally analyzed.
Review Date: 6 Sept 2024 | Illumina
Very useful in my research
Used for de novo sequencing of species without reference genomes
The product is highly efficient and accurate.
Review Date: 23 Jul 2022 | Illumina
Simple to use, easy maintenance, and quick results
DNA Library QC
The HiSeq 2500 is very simple to use, and gives high quality results in a quick turn around time. The system works best when used frequently, and is very easy to maintain. The use of two flowcells means many samples can be QCed at once.
Review Date: 12 Jun 2018 | Illumina
NGS instrument
ChIP-Seq, RNA-Seq, RIP_seq, DRIP-Seq,ATAC-Seq, SeqCapEPI, smallRNA,Amplicon, CRISPR
We achieve really high quality of data and reproducibility. The price of consumables to run flowcell is really high. Advice from Illumina is really rare
Review Date: 23 Nov 2017 | Illumina
Great results, high flexibility, getting more expensive to operate
DNA sequencing
The HiSeq 2500 provides unparalleled flexibility among Illumina's instrument lineup. Many options for high output and rapid runs. The current 4 dye chemistry provides excellent data quality. A patterned flow cell for HiSeq would extend the life of the instrument for many labs. The annual service contract is far too expensive
Review Date: 3 Nov 2017 | Illumina
Flexible, massive output, simple protocol, good after-sale service
Whole genome and targeted DNA sequencing
Very simple and direct protocol, from checking the quality of DNA to running the sequencing libraries in HiSeq. Most importantly, HiSeq offers such flexibility, I can sequence organisms with smaller genome to organisms with huge genome. What I need is to know how much output I need, then I can select Single Flow Cell or Double Flow Cell, high output mode or rapid run mode, then select the read length I prefer, ranging from 1x36bp to 2x125bp. I can even customize the read length I prefer. There is even an online Illumina Sequencing Coverage Calculator. With this calculator, I know what mode to go for, what kit to buy and how deep the coverage of the sample. The output for the HiSeq 2500 is huge. Thus, it has a relatively low cost-per-base. Illumina has this BaseSpace apps, in which you can install into your phone and monitor your sequencing experiment. You can even log into BaseSpace via your computer to monitor your run and even share your experiment monitoring with your colleagues. The after-sale service is good, as they will try they very best to answer my questions. When my sequencing experiments failed due to reagents or instrument issue, the company will replace the kits.
Review Date: 23 Jan 2017 | Illumina
Instrument needed!
Genomics and epigenomics work
Our research would be very difficult or maybe even impossible without use of an illumina sequencer.
Review Date: 17 Jan 2017 | Illumina
Better Chemistry, service and easy use. What else do we require?
DNA sequencing
Yes, it is very easy to use and better Chemistry and the service is good so I recommend this product.
Review Date: 28 Jul 2016 | Illumina
Great Results! Love it!
To analyze Whole exome sequencing data
The results are really fantastic and the technical support provided is top class
Review Date: 29 Jun 2016 | Illumina
Great sensitivity
Sequencing variants
It is easy to use and reproducible for genetic testing
Review Date: 24 Nov 2015 | Illumina
Power and efficiency for large-scale genomics.
The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. No other system lets you perform so many applications so quickly and easily. Choose between rapid run and high output modes offering scalable output to fit your project needs. New reagents enable the generation of up to 1 terabase (Tb) of data in high output mode, supporting the greatest number of samples per run.
Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 150 base pair reads, which enable greater depth of coverage and improved assembly for de novo applications.
High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process > 6× more samples than rapid mode and to complete large projects quickly and efficiently. New HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.
