Products & ReviewLife Sciences

HiSeq 2500 Sequencing System

IlluminaAvailable: Worldwide

Power and efficiency for large-scale genomics. The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. No other system lets you perform so many applications so quickly and easily. Choose between rapid run and high output mode…

Illumina

The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our  Product Directory.

Average Rating 4.4

|13Scientists have reviewed this product

Ease of Use
After Sales Service
Value for Money
Write your own review

Just use it

 

Average Rating 5.0

Application Area:

Whole genome sequence

This is the machine that makes your dreams a reality, as we know DNA and protein I can say the macro molecule are mysterious molecules but through this machine you can notify every activity of that.

Review Date: 19 Sept 2024 | Illumina

Recommend

 

Average Rating 5.0

Application Area:

Bioinformatics

The hiSeq system is novel and enables accurate analysis of RNASeq data (from post mortem human Alzheimer's Disease (AD)/Parkinson's Disease (PD) patients). The data can be computationally analyzed.

Review Date: 6 Sept 2024 | Illumina

Very useful in my research

 

Average Rating 3.7

Application Area:

Used for de novo sequencing of species without reference genomes

The product is highly efficient and accurate.

Review Date: 23 Jul 2022 | Illumina

Simple to use, easy maintenance, and quick results

 

Average Rating 4.3

Application Area:

DNA Library QC

The HiSeq 2500 is very simple to use, and gives high quality results in a quick turn around time. The system works best when used frequently, and is very easy to maintain. The use of two flowcells means many samples can be QCed at once.

Review Date: 12 Jun 2018 | Illumina

NGS instrument

 

Average Rating 3.3

Application Area:

ChIP-Seq, RNA-Seq, RIP_seq, DRIP-Seq,ATAC-Seq, SeqCapEPI, smallRNA,Amplicon, CRISPR

We achieve really high quality of data and reproducibility. The price of consumables to run flowcell is really high. Advice from Illumina is really rare

Review Date: 23 Nov 2017 | Illumina

Great results, high flexibility, getting more expensive to operate

 

Average Rating 4.0

Application Area:

DNA sequencing

The HiSeq 2500 provides unparalleled flexibility among Illumina's instrument lineup. Many options for high output and rapid runs. The current 4 dye chemistry provides excellent data quality. A patterned flow cell for HiSeq would extend the life of the instrument for many labs. The annual service contract is far too expensive

Review Date: 3 Nov 2017 | Illumina

Flexible, massive output, simple protocol, good after-sale service

 

Average Rating 5.0

Application Area:

Whole genome and targeted DNA sequencing

Very simple and direct protocol, from checking the quality of DNA to running the sequencing libraries in HiSeq. Most importantly, HiSeq offers such flexibility, I can sequence organisms with smaller genome to organisms with huge genome. What I need is to know how much output I need, then I can select Single Flow Cell or Double Flow Cell, high output mode or rapid run mode, then select the read length I prefer, ranging from 1x36bp to 2x125bp. I can even customize the read length I prefer. There is even an online Illumina Sequencing Coverage Calculator. With this calculator, I know what mode to go for, what kit to buy and how deep the coverage of the sample. The output for the HiSeq 2500 is huge. Thus, it has a relatively low cost-per-base. Illumina has this BaseSpace apps, in which you can install into your phone and monitor your sequencing experiment. You can even log into BaseSpace via your computer to monitor your run and even share your experiment monitoring with your colleagues. The after-sale service is good, as they will try they very best to answer my questions. When my sequencing experiments failed due to reagents or instrument issue, the company will replace the kits.

Review Date: 23 Jan 2017 | Illumina

Instrument needed!

 

Average Rating 4.0

Application Area:

Genomics and epigenomics work

Our research would be very difficult or maybe even impossible without use of an illumina sequencer.

Review Date: 17 Jan 2017 | Illumina

Better Chemistry, service and easy use. What else do we require?

 

Average Rating 5.0

Application Area:

DNA sequencing

Yes, it is very easy to use and better Chemistry and the service is good so I recommend this product.

Review Date: 28 Jul 2016 | Illumina

Great Results! Love it!

 

Average Rating 5.0

Application Area:

To analyze Whole exome sequencing data

The results are really fantastic and the technical support provided is top class

Review Date: 29 Jun 2016 | Illumina

Power and efficiency for large-scale genomics.

The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. No other system lets you perform so many applications so quickly and easily. Choose between rapid run and high output modes offering scalable output to fit your project needs. New reagents enable the generation of up to 1 terabase (Tb) of data in high output mode, supporting the greatest number of samples per run.

Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 150 base pair reads, which enable greater depth of coverage and improved assembly for de novo applications.

High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process > 6× more samples than rapid mode and to complete large projects quickly and efficiently. New HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.

Product Overview

Links