Histone Demethylase Assay (Fluorescent)
Simple solution for screening activity of purified or recombinant lysine specific demethylase enzymes
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The Histone Demethylase Assay (Fluorescent) provides a simple solution for screening activity of purified or recombinant lysine specific demethylase (LSD1, also known as KDM1) enzymes.
The Recombinant Histone H3K4me2 substrate used in the assay mimics a native histone substrate, allowing for results that more closely resemble in vivo conditions. As the LSD1 enzyme demethylates the histone substrate, formaldehyde is released as a by-product. The Detection Reagent reacts with each formaldeyde molecule to generate a fluorescent signal equivalent to the overall production of formaldehyde. The fluorescent signal can be measured using a fluorescent microplate reader with an excitation wavelength of 410 nm and an emission wavelength of 480 nm.
