Human Peripheral Blood CD4+CD45RO+ T Cells, Frozen
Primary human cells, frozen
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Primary human CD4+CD45RO+ T cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using negative immunomagnetic separation techniques. PB was collected using one of the following anticoagulants: acid-citrate-dextrose (ACD), acid-citrate-dextrose solution A (ACDA), citrate-phosphate-dextrose (CPD), citrate-phosphate-double-dextrose (CP2D), or citrate-phosphate-dextrose-adenine (CPDA). CD4 and CD45RO are co-expressed on memory CD4+ T cells.
Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
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