Human Peripheral Blood Macrophages, Frozen
Primary human cells, frozen
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Primary human macrophages were derived from immunomagnetically selected peripheral blood (PB) monocytes. Monocytes were cultured in RPMI 1640 Medium with 10% fetal bovine serum (FBS), M-CSF and IL-4 for 5 days to generate macrophages. PB was collected using one of the following anticoagulants: acid-citrate-dextrose (ACD), acid-citrate-dextrose solution A (ACDA), citrate-phosphate-dextrose (CPD), citrate-phosphate-double-dextrose (CP2D), or citrate-phosphate-dextrose-adenine (CPDA).
Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
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