Human TNF-alpha ELISA

Assay Format

Detection Range

30 pg/ml - 6000 pg/ml

Recommended Dilution (serum & plasma)

2 fold

Sensitivity

30 pg/ml

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4

Species Detected

Human

Compatible Sample Types

Cell Culture Supernatants, Plasma, Serum

Design Principle

Sandwich-based

Method of Detection

Colorimetric

Quantitative/Semi-Quantitative

Quantitative

Solid Support

96-well Microplate

Protein Information

Accession Number

P01375

Gene ID

7124

Gene Symbols

TNF, TNFA, TNFSF2

Protein Name & Synonyms

Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]

Target Species

Human

Product Features

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.