Hydrophobic interaction resins

Hydrophobic interaction chromatography is a versatile technique that can be used for capture, intermediate purification, or polishing steps. The major advantage of HIC is providing selectivity that unavailable with affinity or IEX resins. HIC is suitable for purification of a large range of molecules:

• Recombinant proteins and peptides
• Monoclonal antibodies (mAbs), bispecific mAbs, and antibody-drug conjugates (ADC)
• mRNA
• Plasmids

HIC is particularly well suited for capture steps after sample cleanup by ammonium sulfate precipitation or for intermediate steps directly after an ion exchange separation where the sample is already in a high-salt solution.

Overview of Capto™ HIC resins

Capto™ HIC resins are based on a rigid, high-flow agarose base matrix with an optimized pore structure that offers good pressure and flow. These resins are intended for use in process development and large-scale manufacturing and in research labs. Their high flow rates allow increased productivity and large-volume processing.

High-flow and high-capacity resins:

• Capto Phenyl resin (high sub)
• Capto Butyl resin
• Capto Octyl resin
• Capto Butyl-S resin

High-resolution resins:

• Capto Phenyl ImpRes resin
• Capto Butyl ImpRes resin

Capto PlasmidSelect

Purified plasmid DNA is required in increasing quantities to meet emerging requirements for gene therapy and DNA vaccine applications. Capto PlasmidSelect resin is a thiophilic aromatic adsorption chromatography resin with a selectivity that allows supercoiled, covalently closed, circular forms of plasmid DNA to be separated from open circular forms. The resin is designed for the purification of high quality, supercoiled DNA for gene therapy and DNA vaccine applications.

Resin selection

To select the most suitable resin for your specific needs, we recommend starting with one these kits:

• PreDictor™ Capto HIC screening kit: a set of 96-well plates prefilled with six Capto hydrophobic interaction chromatography resins for use in high-throughput process development
• HiTrap™ Capto HIC selection kit: a set of five Capto HIC resins in HiTrap columns for resin selection and condition screening

Prepacked formats available for Capto HIC resins

Our resins are available both in prepacked formats and as bulk resins to be packed in columns for use from lab-scale to large-scale GMP manufacturing.