In vitro ADME Protein Binding Platform
Our Protein Binding Platform assesses drug-protein interactions using equilibrium dialysis and ultracentrifugation methods to determine unbound fractions and distribution in biological samples including plasma, blood, tissues, and cells. This essential ADME service evaluates reversible drug transfer between compartments, providing insights into pharmacokinetics, bioavailability, and potential interactions.
In vitro ADME Protein Binding Platform
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Protein binding significantly influences a drug's distribution, metabolism, efficacy, and toxicity, as only the unbound fraction is pharmacologically active and available for clearance. Our platform addresses this by measuring the free and bound drug forms in diverse matrices, such as plasma, tissue homogenate, including brain and liver homogenate, microsomes, and hepatocytes.
The service employs equilibrium dialysis for efficient, cost-effective screening, where drugs diffuse across a semipermeable membrane until equilibrium is reached, separating free from bound fractions. For higher precision, ultracentrifugation sediments protein-bound complexes, minimizing artifacts and offering detailed characterization of interactions. Custom protocols ensure relevance to specific therapeutic areas.
Key features or benefits:
- Versatile samples: Customizable for plasma, blood, tissues, brain homogenate, liver homogenate, microsomes, and hepatocytes to simulate physiological conditions
- Rapid turnaround: Less than 5 days for results
- Expert support: Experienced team provides assay customization, data analysis, and regulatory-compliant reporting
Relevant applications:
- Early drug discovery: Screening for protein binding liabilities to predict ADME properties
- Preclinical ADME profiling: Assessing distribution, bioavailability, and potential drug-drug interactions
- Lead optimization: Minimizing binding-related issues to improve pharmacokinetics and efficacy
- CNS and oncology projects: Evaluating brain penetration via Kbb and tissue binding.
- Regulatory submissions: Generating validated data for IND/NDA filings and safety assessments
