Incucyte® NucLight Green Lentivirus Reagent (EF1α, Puro) - 0.2mL
One vial of Lentivirus reagent (0.2 mL; >1x106 TU/mL) suitable for transducing cells with a non-perturbing nuclear restricted GFP label. Stable cell lines can be created under puromycin selection.
Incucyte® NucLight Lentivirus Reagents enable efficient, non-perturbing, nuclear labelling of living mammalian cells. They are compatible with convenient transduction protocols and enable real-time cell counting and the calculation of cell doubling times.
Incucyte® NucLight Lentivirus Reagents provide homogenous expression of a nuclear-restricted GFP (green fluorescent protein) or mKate2 (red fluorescent protein) in your choice of primary, immortalized, dividing, or non-dividing cells without altering cell function and with minimal toxicity.
These reagents are ideal for generating stable cell populations or clones using puromycin or bleomycin selection.
The Incucyte® NucLight Lentivirus Reagents have been validated for use with the Incucyte® Live-Cell Analysis System and can be combined with the Incucyte® Caspase-3/7 Reagent, Incucyte® Annexin V Reagents or Incucyte® Cytotox Reagents for multiplexed measurements of apoptosis and cytotoxicity alongside cell proliferation in a single well.