InterPlay N-Terminal Mammalian TAP Vector
The InterPlay Mammalian TAP System allows you to recover interacting proteins from mammalian cells. This method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP).
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The InterPlay Mammalian TAP System allows you to recover interacting proteins from mammalian cells. This method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP). Tandem affinity purification yields your tagged protein and interacting proteins using gentle washing and small molecule elution conditions.
The C- and N-terminal mammalian TAP vectors let you clone your gene of interest with the SBP and CBP tags fused to either the C- or N-terminus of your gene.
Features:
- Recover intact interacting protein partners without protease digestion, as SBP and CBP elute easily from their affinity resins
- Exceptionally pure protein for downstream analysis
- Includes all vectors, resins, and buffers needed to conduct a TAP experiment
- Innovative mammalian TAP vector designs
For Research Use Only. Not for use in diagnostic procedures.
