Invitrogen™ Apolipoprotein 5-plex Human ProcartaPlex™ Panel

The Human Apolipoprotein 5-plex ProcartaPlex Panel is a preconfigured multiplex immunoassay kit that measures 5 protein targets using Luminex xMAP technology. ProcartaPlex preconfigured panels are supplied with all the necessary reagents to perform the assay and are validated to ensure reproducibility and consistency. These panels are designed for those who need greater validation for longitudinal studies.

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample
• Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detec¬tion systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

• Reactivity/species: human
• Suitable sample types: serum, plasma
• Sample volume: 25 µL (serum, plasma)
• Reported application: multiplex immunoassay
• Target list: apolipoprotein A1, apolipoprotein A2, apolipoprotein B, apolipoprotein C2, apolipoprotein E
• Bead regions: APO A1: 57; APO A2: 55; APO B: 38; APO C2: 56; APO E: 77
• Assay range (as determined for Lot 1): APO A1: 0.73-3000 ng/mL; APO A2: 0.02-100 ng/mL; APO B: 4.88-20000 ng/mL; APO C2: 0.56-2300 ng/mL; APO E: 0.22-900 ng/mL
• Assay sensitivity (as determined for Lot 1): APO A1: 0.179 ng/mL; APO A2: 0.003 ng/mL; APO B: 4.880 ng/mL; APO C2: 0.373 ng/mL; APO E: 0.061 ng/mL