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Invitrogen™ Countess™ 3 FL Automated Cell Counter

Thermo Fisher ScientificAMQAF2000Available: Worldwide

The Countess 3 FL Automated Cell Counter is both a brightfield and fluorescence cell counter that uses advanced machine-learning image analysis algorithms to deliver accurate cell counts and viability.  The fluorescence capabilities are user-determined with the ability to switch out light cubes and is useful for reporting and measuring fluorescent protein expression, apoptosis, and cell viability using fluorescent reagents.

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Average Rating 4.4

|7Scientists have reviewed this product

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Great addition to a lab

 

Average Rating 4.3

Application Area:

Count cells like PBMC

Easy to use machine, not much maintenance to do which is great. Count cells in seconds.

Review Date: 29 Sept 2022 | Thermo Fisher Scientific

Easy to use and precise. It is a great instrument

 

Average Rating 4.7

Application Area:

In vitro and in vivo samples

The Invitrogen™ Countess™ 3 Automated Cell Counter is a very versatile instrument that allows you to perform cell counting and also to identify gfp labelled cells. We use it daily for our flow cytometry preparations as well as for the generation of our CRISPR/Cas mutants. It is easy to use and very precise. I have had other cell counters before including the previous version of the countess, but this is superb. I highly recommend it for any lab that needs to do cell counting.

Review Date: 4 Feb 2022 | Thermo Fisher Scientific

Great companion for our cell culture facility!

 

Average Rating 5.0

Application Area:

Cell culture

A local sales rep dropped by to give us an on-site demo trial with our samples. Pretty accurate when compared to manual counting, with better robustness.

Review Date: 16 May 2021 | Thermo Fisher Scientific

Lasted 13 months before it died - conveniently just out of warranty.

 

Average Rating 2.3

Application Area:

Cell counting

It was easy to use except that sometimes when powered off it would freeze starting up and need to be restarted a couple of times. Then we turned it off over Christmas and it wouldn't start back up again at all. We've had it for exactly 13 months, so of course it's no longer covered by warranty. A very expensive piece of equipment to only last 1 year and be completely useless due to what appears to be the result of buggy software. Would not recommend.

Review Date: 16 Jan 2019 | Thermo Fisher Scientific

Very useful for our sn and scRNAseq applications.

 

Average Rating 5.0

Application Area:

Cell or nuclei counting

Use this product with the EVOS GFP light cube for cell and nuclei staining, works great and is very useful for our needs.

Review Date: 17 Dec 2018 | Thermo Fisher Scientific

A must for any lab that does considerable cell culture work.

 

Average Rating 5.0

Application Area:

Counting cells

Fantastic, time-saving instrument. Incredibly simple to use. Struggles a little with smaller/primary hematopoietic cells. Fluorescence detection more suited to very highly expressing cells

Review Date: 25 Oct 2018 | Thermo Fisher Scientific

Super easy to use, even for a novice

 

Average Rating 4.3

Application Area:

Cell counting for single cell library prep

Getting accurate cell counts for single cell library prep is essential. Having never performed cell counting before, I was worried about variability and accuracy. This little instrument is incredibly easy to use with no prior experience and appears to be very accurate for our purposes.

Review Date: 3 Nov 2017 | Thermo Fisher Scientific

The Countess 3 FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, fully automated focus and lighting capability, and image analysis software driven by a neural network-based machine-learning algorithm, for rapid assessment of primary and immortalized cell lines. The Countess 3 FL counter is capable of brightfield or fluorescence illumination with three-channel flexibility (brightfield and two optional fluorescence channels) enabling researchers to count cells, monitor fluorescent protein expression, gain insights to apoptotic processes, and measure cell viability.

  • Accurate—eliminates the subjectivity of manual cell counting and user-to-user variability
  • Flexible—accepts up to two EVOS light cubes, user selectable from more than a dozen fluorescent channels
  • Fast—counts live and dead cells, measures viability, reports average cell size, and saves data with a single touch, in about 20 seconds
  • Convenient—requires no cleaning or routine maintenance; high-resolution capacitive touchscreen and simple user interface provide quick startup and requires minimal training

The Countess 3 FL Automated Cell Counter works in two simple steps:

Pipet sample into a slide – choose between the Countess Reusable Slide or the Countess Cell Counting Chamber Slide

Insert the slide into the instrument - Rapid Capture and Auto-Save modes allow cell counting with a single touch. Focus, lighting, and file saving are executed automatically.

Get accurate cell counts

The Countess 3 FL Automated Cell Counter uses trypan blue staining with brightfield illumination combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 × 104 to 1 × 107 cells/mL (the optimal range is 1 × 105 to 4 × 106 cells/mL), which is broader than that of a hemocytometer.

Simple operation with fluorescent application versatility

Operated using an intuitive user interface, automated cell counting with the Countess 3 FL counter gives you the data you need to prepare your sample for the next passage or experiment and allows you to multi-plex with a variety of fluorescent dyes and proteins excited via EVOS light cubes. You no longer need complex, expensive instruments to obtain reliable data.

Transfer data and images to your PC or cloud

Using the USB port on the Countess 3 FL Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. Alternatively, use the Wi-Fi dongle and save results to the cloud for remote access and sharing.

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