Invitrogen™Dynabeads™ Protein A for Immunoprecipitation
Dynabeads™ Protein A are uniform, 2.8 µm superparamagnetic beads with recombinant Protein A (~45 kDa) covalently coupled to the surface. Dynabeads Protein A provide a superior alternative to Sepharose™ resin or agarose resin for immunoprecipitation (IP), and both manual and automated protocols are available. IP in less than 40 minutes High target protein yield with low antibody consumption Very low non-specific bi…
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Look for drug-binding targets
This product is virtually idiot-proof. The detailed protocol that comes with the kit is not only easy to follow, but very adaptable. The dynabeads are also quite durable and can be stored for some time in the refrigerator and re-used, which increases their value.
Review Date: 23 Jul 2018 | Thermo Fisher Scientific
Analyze protein-protein interaction
I've used Dynabeads in order to study the interaction of two proteins and in particular to pull-down enzymatically byotinilated overexpressed protein to identift its interactors and it works great. The use of the magnet make the process of washing much faster and give better results!
Review Date: 20 Feb 2018 | Thermo Fisher Scientific
Dynabeads™ Protein A are uniform, 2.8 µm superparamagnetic beads with recombinant Protein A (~45 kDa) covalently coupled to the surface. Dynabeads Protein A provide a superior alternative to Sepharose™ resin or agarose resin for immunoprecipitation (IP), and both manual and automated protocols are available.
- IP in less than 40 minutes
- High target protein yield with low antibody consumption
- Very low non-specific binding with high signal-to-noise ratio
- No columns, centrifugations, or time-consuming pre-clearing required
- High reproducibility and high throughput compatible with KingFisher™ instruments