Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit
All-in-one vector system for expression of the functional components needed for CRISPR/Cas9 genome editing
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Molecular Genetics
I will recommend product to any molecular scientist and bioinformatics who wants to delete negative genes, deletrous genes in human genome and Livestock genome.
Review Date: 3 Jul 2023 | Thermo Fisher Scientific
Manufacturer's Response
Thank you very much for your feedback! We’re pleased you had a great experience with our product. If we can provide additional support, please contact us at techsupport@thermofisher.com or visit thermofisher.com/geneediting.
The GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells. The linearized GeneArt® CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. The GeneArt CRISPR Nuclease Vector system offers: • Easy-to-design genome engineering system • Affordable, ready-to-use cloning vectors • Enrichment for hard to transfect or difficult to modify cell lines