Invitrogen™ GeneArt™ Precision gRNA Synthesis Kit
Complete system for rapid synthesis of guide RNA (gRNA)
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The Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with TrueCut™ Cas9 Protein v2 for transfection-ready Cas9 protein/gRNA ribonucleoprotein (Cas9 RNP). This Cas9 RNP format, with our TrueCut Cas9 Protein v2, has been tested in a variety of suspension and adherent cell lines with >70% cleavage efficiencies and no indications of toxicity. Resulting gRNA can also be co-transfected with our ready-to-transfect Invitrogen™ CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches. Features of the Precision gRNA Synthesis Kit include: • Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly • High yield (>10 ug) and concentration (>200 ng/uL) of gRNA
