Invitrogen™ ProLong™ Live Antifade Reagent
Decrease photobleaching of fluorescent signal in live cell imaging
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ProLong Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging. When dyes are illuminated, they can degrade (usually permanently), reducing the sample’s fluorescent intensity and leading to the creation of free radical singlet oxygen, an element that can further degrade neighboring dye molecules. ProLong Live Antifade Reagent metabolizes this the free radical singlet oxygen created when fluorescent dyes degrade, which can lead to further degradation of neighboring dye molecules. This, in turn, increasies the signal intensity and duration while keeping the background signal low. ProLong Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours.
Features:
- Photobleach protection in live cell experiments, for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker, LysoTracker, and CellTrackerdyes
- Little-to-no effect on cellular viability, proliferation, or other cellular functions
- Easy to use, just add to the imaging solution or complete cell culture media or buffer, incubate, and image
