Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA. SuperScript RTs are the most highly trusted and widely used…
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My lab buddy
NGS
Excellent product with great result
Review Date: 15 Jul 2024 | Thermo Fisher Scientific
Result was good
RTPCR
Very easy to use and effective results.
Review Date: 4 Oct 2023 | Thermo Fisher Scientific
Expensive, but good results!
Gene expression analsysis
A bit expensive, but easy to use, and high quality results. You save money by avoiding doing many tests and getting technical errors.
Review Date: 21 Mar 2020 | Thermo Fisher Scientific
Reproducible, highly active on structured RNA!
Detection of RNA
Never had a problem in reverse transcription with this enzyme. We typically use the SuperScript™ IV First-Strand Synthesis System with gene-specific primer. Results are always reproducible, even for highly structured RNA-templates. Reportedly SS IV and Maxima H reverse transcriptases give the highest efficiency on the market.
Review Date: 17 Dec 2019 | Thermo Fisher Scientific
Very effective for my constructs
Reverse transcription
I think the SuperScript II Reverse Transcriptase is a useful product that meets the criteria for Molecular Biologuy and efficiently transcribes RNA with great quality. In my experience it doe not differ much from the SS-III which costs more.
Review Date: 13 Jun 2018 | Thermo Fisher Scientific
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
