Products & Review
Invitrogen™ TrueCut™ HiFi Cas9 Protein
High fidelity Cas9 protein engineered to significantly reduce off-target effects while maintaining high editing efficiency
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TrueCut HiFi Cas9 Protein is a high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Features include:
- Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
- Maintains maximum on-target editing efficiency compared to TrueCut Cas9 Protein v2
- High quality—manufactured under strict ISO 13485 quality standards
- Transfection-ready, using either electroporation or lipid-mediated transfection reagents
- Eliminates time-consuming cloning steps
