iScript™ cDNA Synthesis Kit, 25 x 20 µl rxns
25 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water
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Desirable results are obtained by using this cDNA synthesis kit
Liver tissue samples
The cDNA synthesis kit is totally fantastic and easy to use
Review Date: 17 Dec 2021 | Bio-Rad
Easy to use, great yield every time, painless
RT-PCT, qRT-PCR, etc
Very easy to use, works every time, almost effortless. Kit shelf life is extremely long, never has a problem, best bang for the money!
Review Date: 3 Nov 2017 | Bio-Rad
Great for most cDNA for qPCR
cDNA for qPCR
Great kit for the synthesis of cDNA, is very consistent. However, if you are utilizing a small amount of RNA this kit isn't that great. Would recommend a more sensitive RT.
Review Date: 15 Jan 2016 | Bio-Rad
Review Date: 29 Sept 2015 | Bio-Rad
Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.
Key Features and Benefits
- Up to 15 µl of RNA in a 20 µl cDNA synthesis reaction
- Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
- Fast 26 min cDNA synthesis protocol
- Linear range of 1 µg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase
See the iScript cDNA Synthesis Kit product page for more information and documentation.
Related Products
- View all cDNA Synthesis Kits
- Real-Time PCR Supermixes and Kits
- PrimePCR™ PCR Primers & Assays
Related Categories
- PCR and qPCR Reagents
- Real-Time PCR Detection Systems
- PCR Plastic Consumables
- Instrument Validation Tools and Services
Online Resources
- qPCR/Real-Time PCR
- Genomics
