Isopropyl β-D-1-thiogalactopyranoside
Synonym: IPTG, Isopropyl β-D-thiogalactoside
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Great results were obtained. Will continue using this consumable.
Induction of cloned gene expression
IPTG has been very effectively used for inducing protein expression in cloned genes under lac operon. The product has been excellent in achieving the above stated function.
Review Date: 2 Feb 2021 | Merck
Packaging
1, 5, 10 g in poly bottle
Application
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Other Notes
Non-metabolizable galactose analog.
Reconstitution
A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.
