iTaq™ Universal Probes One-Step Kit, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
500 x 20 µl reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water
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Use our combined iScript RNase H+ reverse transcriptase and antibody hot-start iTaq DNA polymerase probe kit formulation to complete your real-time reactions in one step — PCR efficiency is improved over a wide dynamic range and under various conditions.
- Advanced formulation for amplifying difficult target sequences and challenging samples
- Perform singleplex or multiplex reactions simultaneously
- Normalize qPCR data with ROX normalization dyes
Kit Components
- Reverse transcriptase — 250 µl
- Reaction mix — 5 ml (5 x 1 ml)
Instrument Compatibility
iTaq Universal Probes One-Step RT-qPCR Kit is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
* BSA must be added according to instrument specifications
Applications and Uses of the iTaq Universal Probes One-Step Kit
- qPCR/real-time PCR
- Gene expression analysis
- Multiplexing
- Absolute quantification
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Genetically modified organisms (GMO) characterization
- Genetic profiling
The iTaq universal probes one-step kit is also available in a format of 100 reactions ( 1725140 ).
Related Products
Bio-Rad has also developed one-step kits for SYBR® Green–specific applications .
