IWP-2

IWP-2 inhibits the WNT pathway (IC₅₀ = 27 nM) at the level of the pathway activator Porcupine. Porcupine is a membrane-bound acyltransferase that palmitoylates WNT proteins, which leads to WNT secretion and signaling capability. (Chen et al., Willert et al.)

DIFFERENTIATION

• Suppresses self-renewal of mouse embryonic stem (ES) cells and supports their conversion to epiblast-like stem cells (ten Berge et al.).
• Inhibits maintenance and proliferation of mouse Lgr5+ intestinal and cochlear epithelial stem cells, demonstrating the importance of WNT signaling in these processes (Chai et al., Farin et al.).
• Promotes cardiomyocyte differentiation from human pluripotent stem cells (Lian et al., Minami et al.).