KAPA Rapid Ligation System
Fast and efficient ligation. The KAPA Rapid Ligation System is a combination of KAPA Rapid T4 DNA Ligase and an optimized reaction buffer designated to ligate blunt or cohesive end DNA fragments in 5 minutes at room temperature. KAPA Rapid Ligase contains T4 DNA Ligase, which catalyzes the formation of phosphodiester bond between 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. Features: 5-minute ligati…
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Fast and efficient ligation.
The KAPA Rapid Ligation System is a combination of KAPA Rapid T4 DNA Ligase and an optimized reaction buffer designated to ligate blunt or cohesive end DNA fragments in 5 minutes at room temperature. KAPA Rapid Ligase contains T4 DNA Ligase, which catalyzes the formation of phosphodiester bond between 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA.
Features:
5-minute ligation
- Joins blunt- and cohesive-end DNA in only 5 minutes
- Repairs single-stranded nick in duplex DNA, RNA, or DNA/RNA hybrids
Convenient-use format
- Ligation is performed at room temperature
- Kit contains all buffers and enzymes needed
Applications:
- Routine subcloning
- Recircularization of linear DNA
- Library construction
- Linker ligation
