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Keratin K18, bovine, 100 µg

PROGEN62204

Protein standard for immunoblotting, immunization and immunoassays.

PROGEN

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Protein standard for immunoblotting, immunization and immunoassays.

Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).

For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

  • Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
  • Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255

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