Keratin K8, human recombinant, 100 µg

Human recombinant Keratin K8 for use in immunoblotting and ELISA.

Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).

For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

• Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
• Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255