KRIBIOLISA™ Nivolumab (OPDIVO) ELISA
The Nivolumab ELISA kit is an assay intended for the quantitative determination of Nivolumab in serum, plasma and cell culture samples.
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OPDIVO® (Nivolumab) is a prescription medicine used in combination with YERVOY® (ipilimumab) as a first treatment for adults with a type of advanced stage lung cancer (called non-small cell lung cancer) when your lung cancer has spread to other parts of your body (metastatic) and your tumors are positive for PD-L1, but do not have an abnormal EGFR or ALK gene.
The method employs the quantitative sandwich enzyme immunoassay technique. Antibodies to Nivolumab are pre-coated onto microwells. Samples and standards are pipetted into microwells and human Nivolumab present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-nivolumab antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Nivolumab in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
- Calibrator Range: 0 ng/ml - 640 ng/ml
- Sensitivity: 6 ng/ml
- Sample Type: serum and plasma
- Shipping Condition: 2 - 8 Deg C
- Detection Method: Colorimetric
- Usage: For Research Use Only
- Research Area: Immune Checkpoints Therapeutic Drug Monitoring Cancer
Krishgen kits have over 1000 citations, with our ELISA kits being used for publications in 15+ countries.
