Lambda EMBL3/Gigapack III Gold Cloning Kit
The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection. High-efficiency lambda cloning with versatili…
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The Lambda ZAP-CMV vector allows production of libraries in a high-efficiency lambda vector. Libraries cloned into lambda vectors routinely produce greater than 4 times the primary library size as plasmid libraries. The Lambda ZAP II system combines the high efficiency of lambda library construction and the convenience of a plasmid system with improved blue-white color selection.
- High-efficiency lambda cloning with versatility of plasmid
- in vivo excision of cloned fragments into phagemid vector
- Expression of fusion proteins under control of lac promoter
- Allows screening in lambda with DNA probe or in mammalian cells with functional screen
- Create large, highly-representational libraries
- Clone genes that are toxic in E.coli
