Products & ReviewLife Sciences

Live/Dead Cell Viability Assay Kit (for Mammalian Cells)

BioVision IncAvailable: Worldwide

This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis. The kit provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes.  Live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluore…

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Average Rating 4.8

|2Scientists have reviewed this product

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Very helpful.

 

Average Rating 4.7

Application Area:

Biology

Very useful to evaluate cell viability in 2D and 3D engineered tissues.

Review Date: 10 Feb 2020 | BioVision Inc

Excellent product . I got good publication standard images of my samples.

 

Average Rating 5.0

Application Area:

tissue engineering

I am using this kit from Last 7 years. It is very easy to use and give excellent results. Cost is also reasonable. Good product.

Review Date: 23 Jul 2018 | BioVision Inc

This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis. The kit provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes.

Live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm.

Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells.

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