Long Oligos
Oligos from 121 to 180 bases
Virology
It was easy to place orders. List price is a bit high but nothing crazy. Communication when primer takes longer than expected is poor have to chase and get an answer several days later. But usually deliver whats promised and provide extra labels and still include a paper copy of QC, very much appreciated. No shipping charge!
Review Date: 17 Feb 2022 | Merck
Genotyping and education
My lab sometimes needs designed oligos for genotyping experiments with high-school students. Actually I hesitated to order a long oligo once, because I felt it might be expensive and not be high enough quality. But that was a pointless concern. The long oligo from Merck worked very well and was cost effective, so we could establish a procedure to recognize a SNP genotype with normal agarose gel. Now we are preparing for a coming delivery-lecture in near future (maybe after coronavirus pandemic).
Review Date: 31 May 2021 | Merck
Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long-oligo synthesis. The techniques we have developed to purify long oligos are unmatched by other suppliers.
- Bases: DNA (A,C,G,T) and degenerate bases
- Backbone: Phosphodiester & phosphorothioate
- Length: 121 - 180 bases
- Purification & Yield: Cartridge (5 OD) & PAGE (0.5 OD)
- Modification: 5' end (Phosphate, Biotin, Amine C6, Amine C12, Inosine, & deoxyUracil), 3' end (Spacer C3), & Internal (Inosine & deoxyUracil)
- Format: Dry in 2 mL screw cap tubes
- Documentation: Delivered with Quality Assurance document including yields, Tm, MW, & µg/OD
- Quality Control: Every oligo is quality checked by mass spectrometry or PAGE & OD by UV Spectroscopy
- Shipping Schedule: Cartridge & PAGE: 10 business days