Macrophage Generation Media XF

The PromoCell Macrophage Generation Media were developed for easy and efficient generation of the full spectrum of M1- and M2-polarized macrophages directly from freshly isolated peripheral blood monocytes.

The PromoCell M1-Macrophage Generation Medium xf contains GM-CSF and allows for the generation of M1-polarized macrophages, whereas the M2-Macrophage Generation Medium xf contains M-CSF and produces M2-polarized macrophages. Both media are ready-to-use and tested in-house for successful differentiation of M1-/M2-macrophages via flow cytometry.

The Macrophage Base Medium xf is the user-customizable version of the Macrophage Generation Media xf and does not include cytokines. It comes without cytokines as a universally applicable MDM culture system featuring a fully user-customizable macrophage differentiation and activation process.

The Monocyte Attachment Medium allows for the efficient adherence of fresh monocytes as well as for adherence selection of monocytes from freshly isolated Mononuclear Cells (MNC) while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes prior to macrophage differentiation is not necessary.

All PromoCell Macrophage Generation Media xf are defined and xeno-free media formulations, free of all animal-derived components and substances of human origin with Human Serum Albumin as the only exception.


Macrophage Generation Media xf Advantages:

• Easy access to the full range of macrophage activation states
• Obtain fully functional, untouched macrophages directly from peripheral blood mononuclear cells
• Generate macrophages from up to 5 buffy coats per medium bottle within one week
• No time-consuming and costly immunomagnetic purification of monocytes necessary
• No immunogenic or deleterious effects from animal-derived components