MegaMix Emerald - qPCR Dye Based

Containing all the components needed to perform qPCR swiftly and reliably.

The 2X mix contains chemically modified Hot Start Taq DNA polymerase, intercalating dye, dUTP and UNG in enhancing buffer optimised for amplifying low copy DNA targets. The third generation intercalating fluorescent dye binds to double stranded DNA, making MegaMix Emerald the perfect choice for qPCR, Melt Curve Analysis and High-Resolution Melting (HRM). The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity, eliminating the formation of non-specific amplification and primer-dimers. The presence of UNG and dUTP’s eliminates carryover contamination, the thermolabile UNG is active at room temperature before being completely and irreversibly inactivated when heated to 95°C, this means your PCR product is suitable for downstream processing.

Key Features

• Specificity—Hot Start Taq DNA Polymerase in optimized buffer eliminates non-specific amplification and the formation of primer dimers.
• Sensitivity—Single copy detection.
• Eliminates carry over contamination—incorporation of thermolabile UNG and dUTP prevent amplicon contamination from previous runs without degrading generated amplicons.
• Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
• Reproducibility and convenience—ready to use 2X format.
• Inhibitor Resistance—suitable for direct to PCR with products such as microLY-SIS-PLUS and microLYSIS-RNA.
• Third generation intercalating dye— no inhibition of PCR, even at high concentrations, suitable for High Resolution Melting.

Applications

• Quantification of any DNA template (gDNA, cDNA, viral DNA), low copy number samples.
• Genotyping assays.
• Gene expression analysis.
• Pathogen detection.
• qPCR assays using fluorescence of intercalating dye.
• High resolution melting.