MegaMix Platinum - qPCR Probe Based
Reliable multiplex qPCR kit. Optimised for amplifying low copy DNA targets. Ideal for multiplexed reactions of 4 or 5 targets without compromising performance.
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Containing all the components needed to perform multiplex qPCR swiftly and reliably.
The 2X mix contains chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in Microzone’s proprietary enhancing buffer optimised for amplifying low-copy DNA targets. The presence of thermolabile UNG and dUTP eliminates carryover contamination at room temperature. Unlike other UNG enzymes, it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis. This mix has been optimized for multiplex assays utilizing dual labeled hydrolysis probes and is compatible with both standard and fast cycling conditions.
Key Features
- Multiplexing—excellent for multiplexed reactions consisting of 4 or 5 targets without compromising performance.
- Eliminate carry over contamination—incorporation thermolabile UNG and dUTP prevent amplicon contamination from previous runs.
- Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
- Inhibitor Resistance—suitable for direct to PCR with products such as microLYSIS-PLUS and microLYSIS-RNA.
Applications
- Multiplexed probe based assays including TaqMan molecular beacons, ScorpionsTM probes.
- Quantification of any DNA template (gDNA, cDNA), low copy number samples.
