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Mobilized Human Peripheral Blood CD34+ Cells, Frozen

Primary human cells, frozen

STEMCELL Technologies Inc.

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Primary human CD34+ cells were isolated from mobilized peripheral blood (PB) leukapheresis samples using positive immunomagnetic separation techniques. PB was collected using acid citrate dextrose solution A (ACDA) as an anticoagulant. Donors were mobilized with:

  • Catalog #70060: a maximum of 10 µg/kg/day of granulocyte colony-stimulating factor (G-CSF) for 3 - 5 days prior to collection
  • Catalog #70075: a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection
  • Catalog #70073: a maximum of 10 µg/kg/day of G-CSF for 3 - 5 days prior to collection and a maximum of 0.24 mg/kg of plerixafor for 1 day prior to collection

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.

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