Monkeypox Virus (MPXV) PCR Nucleic Acid Detection Kit
The RayBiotech Monkeypox Virus (MPXV) PCR Nucleic Acid Detection Kit is a ready to use PCR assay for the detection of MPXV DNA in a liquid sample. It uses a fluorescent probe with specific primer sets to detect the J2R gene specific for the genome of MPXV.
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While Monkeypox viruses (MPXV) are usually zoonotic diseases (generally transmitted from animal to human), this MPXV outbreak is seeing higher levels of presumptive human to human spread for reasons that are currently not well understood. This new MPXV isolate is nearly identical to a recent outbreak in the UK, differing by less than 100 bases out of over 197,000 nucleotides. RayBiotech developed this Monkeypox Virus PCR Kit as a ready to use PCR assay for the detection of MPXV DNA to help the research community better test, evaluate, and understand this current viral strain. It does this by using primer sets specific for the J2R gene unique to the current strain of circulating MPXV (GenBank: ON563414.3) and can be measured with most common qPCR setups.
Product Features:
- Monkeypox virus specific / no cross-reacitvity with other Orthopox viruses.
- Limit of Detection: 4 copies / µL in the reaction
- Repeatability: Intra-assay %CV is ≤ 1.2%; Inter-assay %CV is ≤ 10%
- Hot-start Taq DNA Polymerase with high efficiency and fidelity.
- Double quencher probe ensures enhanced specificity.
- UDG and dUTP system minimizes carry-over contamination.
- ROX reference dye improves accuracy and reproducibility.
