NanoBRET™ PPI Starter Systems
BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells
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Sensitive, Scalable Assays to Study Protein Interactions in the Natural Cellular Context
NanoBRET™-based protein:protein interaction (PPI) assays use NanoLuc® Luciferase as a BRET energy donor and HaloTag® protein, labeled with the NanoBRET™ 618 fluorophore, as the energy acceptor to measure the interaction of specific protein pairs.
The NanoBRET™ PPI Starter Systems provide the vectors required to create NanoLuc® Luciferase and HaloTag® protein fusions to target proteins of interest, a Positive Control Pair (p53, MDM2) and the NanoBRET™ Nano-Glo® Detection System, which contains the substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag® NanoBRET™ 618 Ligand for the fluorescent energy acceptor.
- Live-cell reagents allow you to detect protein:protein interactions in real time using full-length proteins or fragments
- Reversible assay technology allows you to study both induction and inhibition of protein interactions
- Assays can be performed in 96- or 384-well formats with low variability and high reproducibility
