NanoBRET™ Target Engagement BET BRD Assays
The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells
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The nanoBRET assay is set-up for several projects in our company and we recommend it!
evaluate compounds for their inhibitory activity
The nanoBRET assay has the additional advantage of using a ratiometric readout, allowing intra-well normalization and showing increased assay quality. It enables medium/high quality results depending on the constructs used. It makes the link between in vitro and in vivo assays.
Review Date: 16 Apr 2018 | Promega Corp.
The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells
