Neon Nxt Electroporation System
Fast and efficient delivery of nucleic acids
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After electric shock my cell still alive and expressing my target.
Transecting large plasmid and hard to transfect gene
This system is highly efficient with high cell viability and transfection efficiency for large size plasmids.
Review Date: 17 May 2024 | Thermo Fisher Scientific
Manufacturer's Response
Thank you for taking the time to share your feedback on the Neon NxT Electroporation System. We appreciate your kind words and are delighted to hear that you find our system highly efficient, with high cell viability and transfection efficiency for large size plasmids.
Electroporate cells like magic
electroporation of Hematopoietic stem cells
Instrument is perfect for electroporation of primary cells such as hematopoietic stem cells.
Review Date: 30 Jun 2023 | Thermo Fisher Scientific
Manufacturer's Response
Thank you for sharing your positive experience with our Neon NxT Electroporation System. We are always eager to help our customers with continued product support. Please contact us at customercare@thermofisher.com. Cell specific neon protocols available under our protocol & citation library would also be a helpful resource for your optimization experiments. Wishing you continued success with your transfection goals!
Fantastic electroporator with high transfection efficiency and high cell viability
Cell transfection
Relatively easy to use once you figure out how to attach the special tips onto the Neon Pipette. I managed to achieve a high transfection efficiency while maintaining a high viability with the Neon Transfection system.
Review Date: 10 Feb 2023 | Thermo Fisher Scientific
Manufacturer's Response
Thank you for sharing your positive experience with our Neon Transfection System. We are always eager to help our customers with continued product support. Please contact us at customercare@thermofisher.com. Cell specific neon protocols available under our protocol & citation library would also be a helpful resource for your optimization experiments. Wishing you continued success with your transfection goals!
we get great results, and forget the other transfection methods.
Cell biology, molecular biology
This is an electroporation system, which was really effective according to our results. It is easy to use, the protocol is clear and not complicated, the method is quick. The transfection effeciency was higher than 60-70%, compared to the Lipofectamine method. The only reason why it can not fit for you is the high price of the consumables, however it is worth it
Review Date: 6 Feb 2023 | Thermo Fisher Scientific
Manufacturer's Response
Thank you for sharing your positive experience with our Neon Transfection System. We are always eager to help our customers with continued product support. Please contact us at customercare@thermofisher.com. Cell specific neon protocols available under our protocol & citation library would also be a helpful resource for your optimization experiments. Wishing you continued success with your transfection goals!
Easy and convenient!
Transfection
Very convenient and easy to use! With minimum cell death, it allows our experiment to be much more efficient!
Review Date: 18 Jan 2021 | Thermo Fisher Scientific
Efficient and quick transfection.
Transfection
As our focus is on gene editing and we need high transfected cells, this device helps us to get up to 90% efficiency in even untransferable cells with minimal cell death. This kit can be used for all cell types and also for DNA, RNA and protein.
Review Date: 8 Jul 2020 | Thermo Fisher Scientific
Efficient, quick and easy.
Electroporation
This system is very efficient, quick and easy. It shows high transfection efficiency even for difficult cells e.g. primary stem cells. Operation is very user friendly and all the kits are easily available and simple to use. It is equally good for all cell types.
Review Date: 21 Jun 2020 | Thermo Fisher Scientific
Perfect system for the Transfection
Transfection
Neon Transfection System is way easier when going for the transient transfection method and the electroporation makes the DNA enters the cells instant and makes the transfection easier. Some transfection reagents may kill the cells when it exceeds the recommended but the neon transfection method works perfect.
Review Date: 30 Oct 2017 | Thermo Fisher Scientific
The Neon Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability.
- Performance — Achieve >90% efficiency and >95% cell viability in over 150 mammalian cell lines
- Flexibility— Precisely optimize electroporation parameters to deliver DNA, RNA, or protein in as little as 2 x 10⁴ cells to as much as 6 x 10⁶ cells per reaction.
- Time-saving— Plug-n-play out of box, and transfect cells quickly with 3-step workflow, a single buffer kit and intuitive user interface
- Preserve sample — Minimize sample transfer loss and contamination risk of valuable cells with unique and compact electroporation instrument design
