Nikon Microscope with Yokogawa CSU-W1 SoRa Spinning Disk Confocal
Spinning Disk Super Resolution at high speed.
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The CSU-W1 SoRa is a Spinning Disk Super Resolution system using Optical Pixel Reassignment.
Super-resolution images are acquired optically, on any sample, without special preparations or computation. This allows image collection to be limited only by the sample’s signal-to-noise ratio and the exposure time of the detector. Video rate or faster super resolution imaging is possible.
Microlensing the emission pinhole at the correct magnification counteracts the mismatch between an on-axis excitation PSF and the effective confocal PSF (product of excitation and emission PSFs) through an emission pinhole that is not infinitely small.
By microlensing, individual points’ divergence angle onto the pinhole is reduced 2x, mimicking the effect of an infinitely small ideal pinhole, but not compromising signal brightness.
Utilizing magnification and microlensing, an approximately 1.4x improvement beyond the optical limit is achieved, which can further be improved by deconvolution to 2x. This allows for XY resolution up to 120nm.
