NTA Agarose (25 ml)

Uncharged NTA Agarose allows researchers to choose the metal ion they use for IMAC procedures, allowing fine-tuning of purification strategies. Metal ions such as Ni ²⁺, Cu ²⁺, Zn²⁺, or Co²⁺ are efficiently immobilized for IMAC purification of metal-binding proteins. NTA has four chelating sites to bind metal ions more tightly than other metal-chelating purification systems. This reduces metal-ion leaching and nonspecific binding and leads to increased protein binding capacity and purer preparations. A proprietary spacer that links NTA to the sepharose support provides optimal accessibility to metal ions, increasing protein-binding capacity.