NucleoCounter® NC-202™ automated cell counter
The NucleoCounter® NC-202™ automated cell counter is our 3rd generation cassette-based image cytometer. Featuring improved hardware and new NC-View™ software, we take precision, speed and data management to new levels.
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Excellent instrument: accurate, robust, and easy to use.
Engineered CHO cell culture characterisation
The instrument was used to monitor CHO cell cultures in an academic setting. The cells often expressed fluorescent proteins as part of our cell experimental workflow. We speculated that there was a possibility of interference between fluorescent protein expression (especially mOrange) and the viability measurements returned by the NC-202 Nucleocounter. We were very pleased to see that fluorescent protein expression had no impact on the accuracy of the viability measurements. Moreover, we had the opportunity to benchmark the NC-202 with manual counting and another automated cell counter from a different provider. The accuracy in quantifying cell density and viability of all our CHO cell cultures was comparable across all methods. Importantly, the ease of use of use of the NC-202 exceeded that of the other two methods, as it did not require dilution or further sample handling. Overall, we were very impressed by the NC-202's performance! I also want to mention that engagement from the ChemoMetec team (commercial and technical) was exceptional! Very rapid response times and genuine interest in assisting in my group's work. Thank you!
Review Date: 5 Nov 2025 | ChemoMetec
Reliable results, no matter the sample!
Vaccine research (immunology, target discovery, vaccine development)
At Infinitopes, precision really matters to us — not only in our target discovery but also in cell counting which is imperial for producing our vaccine vectors and their quality assessments, to testing how our epitopes trigger immune responses. Accurate ad consistent cell counts are key to keeping our research reliable and our data solid. We have used the NC-202 across a range of cell types — HEK293, A549, A375, CaSki, SHP77, and various primary cells — and it’s handled them all with no problems. For adherent cell lines, once they’re trypsinised, counting is straightforward. But even when we skip trypsinisation and leave cells in their natural, sometimes clumpy state, the NC-202 still delivers. Its lysing solution has been a game-changer for getting accurate counts where trypsinisation was not possible for our antigen discovery experiments or when manual methods were to laborious. Compared to the multiple other machines we’ve trialed, the NC-202 stand out first. Its software does an excellent job of recognising and counting cell clumps, and the lysing solution really solves any aggregation issues. We’ve also had great results with PBMCs, which are notoriously tricky due to their size and shape differences. The dedicated PBMC protocol has given us counts that match manual accuracy and since the system is 21 CFR Part 11 compliant it means that is suitable for our clinical trial work that is underway. Overall, the NC-202 has proven incredibly versatile and dependable. Whatever type of sample we’ve thrown at it, it’s delivered accurate, reproducible results - usually you can't have both and there's a trade-off in between the two, but not in this case.
Review Date: 3 Nov 2025 | ChemoMetec
Essential to daily operations
Bioprocess development - Microcarriers & Single cell suspensions
My team uses this instrument on a daily basis. We predominantly do upstream bio-process development for our own products as well as customer projects on viral vaccines, AAV, MSCS and iPSCs. Spinner flasks and stirred tank bioreactors provide a significant challenges in establishing a process, with a cornerstone in the cell biology and cell counting. The NC202 reduces the subjectivity experienced with other bench-top cell counting methods and instruments. After initial cell-specific protocol development, internal and external technology transfer has one less barrier. Every biotech and biopharma we work with, uses a NucleoCounter. Ease of use and training on this technology is another bonus. Biotechnology should take a lesson from the justice system: "Eye-witness testimony is the most flawed, but catching the events on a CCTV..." The above strongly offsets the cost and waste generation from the cartridges if there are high sample numbers to analyze.
Review Date: 8 Apr 2025 | ChemoMetec
Great product, great support
Enumeration or primary cells
Great device, it is fast, reliable and easy to use. The level of support ChemoMetec provide is second to none. Highly recommended.
Review Date: 24 Jan 2025 | ChemoMetec
Great product
Cell counting
Great product, great customer service!
Review Date: 23 Jul 2024 | ChemoMetec
Very good counting device, don't want to use another one anymore.
Cell counting human PBMCs and cell lines
Easy to use and fast. Highly reproducible results. Gives many parameters of counting results e.g. Total cell counts, viable cells, dead cells, vitality of cultured cells which are very helpful for documentation.
Review Date: 6 May 2024 | ChemoMetec
Great system, we use it daily and recommend to all of our clients
Cell therapy
The NC202 has simplified our cell count and viability processing, enabling smooth comparison between process development and GMP. The Chemometec team have been very supportive throughout our development and transition between the NC200, 250 and 202.
Review Date: 2 May 2024 | ChemoMetec
Great results, highly recommended
Cell counting
Equipment of high effectiveness and great customer support. We obtain high quality and reproducible results, very easy to use.
Review Date: 26 Apr 2024 | ChemoMetec
Insect cells counting
Counting Insect cells cultivation
We used the cell counter for insect cell cultures growing in flasks. The counting method works well for this cell type. We obtained consistent and reproducible results even with high cell densities and some cell clusters. The use of cartridges enables safe analyses for the operators.
Review Date: 17 Apr 2024 | ChemoMetec
Makes the day in the lab much faster
Cell counter
Nice and easy to use.
Review Date: 5 Apr 2024 | ChemoMetec
The NucleoCounter® NC-202™ automated cell counter is our 3rd generation cassette-based image cytometer. Featuring improved hardware and new NC-View™ software, we take precision, speed and data management to new levels. The NucleoCounter® NC-202™ is the most precise automated cell counter on the market: The combined inter-operator/inter-instrument variation is typically 5 – 10%. Consequently, you obtain identical results with any cell sample, recorded by any user, located anywhere in the world.
The NucleoCounter® NC-202™ has one universal cell counting protocol that handles all cell types: No optimization is required. The instrument provides robustness and precision regardless of cell size, morphology and media type, even in the presence of red blood cells, magnetic beads or cellular debris. Consequently, the NucleoCounter® NC-202™ is easy to validate, even for the most complex processes.
The all new DebrisIndex™ is a measurement of culture debris build-up. Debris negatively affects downstream filtering and purification steps, so a direct measurement of debris facilitates better process development parameters. The NC-View™ software is compatible with 21 CFR Part 11/GMP guidelines and can be integrated into your clinical workflow.
Our Field Application Scientists (FASs) provide training, service visits, installation and user guidance on-site or online to ensure you get the best start to working with the NucleoCounter® NC-202™.
What’s NEW – NucleoCounter® NC-202™:
- Automated cell counter
- Analysis time only 30 seconds
- Improved light sources for greater signal-to-noise ratio and dynamic range
- DebrisIndex™ monitors cell culture health and bioreactor production runs
- Broader linearity range: Count samples up to 1×107 cells/ml
- Instrument Service Plan: Keep your instrument up-to-date
- Automatic data quality checks for sample artefacts
- Electromechanical cassette slot for easy operation
