One-Step Blue Protein Gel Stain

One-Step Blue^® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue^® is a great choice for convenience and safety. One-Step Blue^® can detect as little as approximately 10-20 ng of protein per band.

• One-step staining procedure, no fixation required
• Non-toxic formulation is safe for easy disposal
• Sensitive protein detection down to 10-20 ng
• Visualize blue bands, or image with a near-infrared fluorescence imager
• 1 L size
• Store at 4°C

Fast and simple procedure

One-Step Blue^® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue^® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence.

One-Step Blue^® is non-toxic

One-Step Blue^® offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

SimplyBlue is a trademark of Thermo Fisher Scientific.