One-Step Blue Protein Gel Stain
A visible, blue protein gel staining solution. Fast protein staining in a single step without fixation or washing.
Colorimetric or fluorescence gel imaging
One-Step Blue (OSB) comparison
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One-Step Blue® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue® is a great choice for convenience and safety. One-Step Blue® can detect as little as approximately 10-20 ng of protein per band.
- One-step staining procedure, no fixation required
- Non-toxic formulation is safe for easy disposal
- Sensitive protein detection down to 10-20 ng
- Visualize blue bands, or image with a near-infrared fluorescence imager
- 1 L size
- Store at 4°C
Fast and simple procedure
One-Step Blue® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence.
One-Step Blue® is non-toxic
One-Step Blue® offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.
SimplyBlue is a trademark of Thermo Fisher Scientific.
