PCR Polishing Kit
PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.
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PCR for DNA extraction to elongate the fragments of the extracted DNA
Excellent for performing polymerase chain reaction to elongate the extracted DNA.
Review Date: 18 Feb 2021 | Agilent Technologies
Environmental
It is very good and very easy to use
Review Date: 26 Mar 2019 | Agilent Technologies
PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.
Dramatic increases in the population of blunt-end DNA fragments following polishing with Agilent’s PCR Polishing Kit enables significant increase in overall experimental efficiency associated with procedures utilizing blunt-ended ligation.
Features:
- Generates blunt-ended DNA fragments in 30 minutes
- More efficient than Klenow polishing
- Optimizes blunt-end cloning efficiencies
For Research Use Only. Not for use in diagnostic procedures.