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PCR Polishing Kit

PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.

Agilent Technologies

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Average Rating 4.5

|2Scientists have reviewed this product

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Great result.

 

Average Rating 5.0

Application Area:

PCR for DNA extraction to elongate the fragments of the extracted DNA

Excellent for performing polymerase chain reaction to elongate the extracted DNA.

Review Date: 18 Feb 2021 | Agilent Technologies

Good.

 

Average Rating 4.0

Application Area:

Environmental

It is very good and very easy to use

Review Date: 26 Mar 2019 | Agilent Technologies

PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.

Dramatic increases in the population of blunt-end DNA fragments following polishing with Agilent’s PCR Polishing Kit enables significant increase in overall experimental efficiency associated with procedures utilizing blunt-ended ligation.

Features:

  • Generates blunt-ended DNA fragments in 30 minutes
  • More efficient than Klenow polishing
  • Optimizes blunt-end cloning efficiencies

For Research Use Only. Not for use in diagnostic procedures.

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