PEG/Ion
PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules. The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a p…
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Proteins Crystallization And Solubility
PEG ION SCREEN 1 and 2 formulated by Hampton Research are good for screening the initial crystallization. It is also useful for finding a good solubility solution for the protein of interest. The two screens together contain 96 solutions with different concentration of salts and also with varying pHs. The PEG ION SCREEN 1 contains PEG 3350 and salts, The pH of which has not been adjusted. PEG ION SCREEN 2 contains PEG 3350 and salts the pH of which have been adjusted (3-8.5). Besides crystallization these screen provide an excellent opportunity to find a good buffer for the protein of interest. I recommend these screens to be used for both crystallization and checking solubility of the protein of interest.
Review Date: 8 Jan 2014 | Hampton Research
PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules.
The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.
PEG/Ion Features:
- PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 - 9.2
- PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 - 8.8) in the presence of monodisperse PEG 3,350
- PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block
- PEG/Ion, PEG/Ion 2 and PEG/Ion HT were developed at Hampton Research
