pGEM®-T Easy Vector Systems
The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products. They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the…
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Great results
Cancer Research
Very easy cloning system with reproducible results
Review Date: 24 Oct 2022 | Promega Corp.
Using the product on a daily basis because of its best performance.
Vector construction for gene isolation.
The pGEM-T Easy vector is linearized with - A single 3´-terminal thymidine at both ends. - The T-overhangs at the insertion sites improve the efficiency of ligation - Insertional inactivation of the α-peptide allows identification of recombinants by blue/white screening on indicator plates. - Contain numerous restriction sites within the multiple cloning region. - Multiple cloning region is flanked by recognition sites for the restriction enzymes - A double-digestion may be used to release the insert from either vector. - An overnight incubation at 4°C produces the maximum number of transformants
Review Date: 13 Feb 2022 | Promega Corp.
Great product.
Molecular biology
Reliable system for molecular cloning that works every time. Highly recommended.
Review Date: 21 May 2021 | Promega Corp.
Our 'go to' for cloning
Cloning metastasis genes for expression and sequencing analysis
The pGEM-T easy vector system is one of the best products we use for cloning. It has high efficiency for the genes we work with and purchase the system with the competent cells as we are guaranteed results. The step by step protocol is easy to follow and is quick. We have also managed to reproduce the results and use the same methods for many genes.
Review Date: 27 Jun 2019 | Promega Corp.
Great result, very quick and easy to use.
Checking PCR
Very easy to use, clear information and instructions are super easy to follow. Gave exactly the results that were expected, and very simple to use to check PCR products. Would not use any other product.
Review Date: 27 Jun 2019 | Promega Corp.
Great product in cloning
Cloning and Sequencing
The pGEM-T easy vector system is one of the best product in cloning with high efficiency. I prefer to buy them along with competent cells, even 1/4 of the recommended amount of competent cells works well. I tried many cloning kits but feel this is the best one in term of repeatability of results
Review Date: 21 Jul 2018 | Promega Corp.
I am fond of this product
Cloning and sequencing
This is really the product which has high efficiency and reproducibility. I suggest buying it alongside competent cells. It works great with very little chance of calibration
Review Date: 27 Feb 2018 | Promega Corp.
Review Date: 2 Feb 2010 | Promega Corp.
The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products.
They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the pGEM(R)-T Easy Vector System I components.
pGEM®-T Easy Vector Systems Features & Benefits:
- Flexibility - The multiple cloning site is flanked by restriction enzyme sites for BstZI, NotI and EcoRI, allowing three options to remove the insert with a single digest.
- Rapid Ligation - The 2X Rapid Ligation Buffer provided allows reactions to be completed in 1 hour at room temperature.
- Blue/White Screening - T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the α-peptide coding region for β-galactosidase. Insertional inactivation of the α-peptide allows recombinant clones to be directly identified by color screening on indicator plates.
- f1 Origin of Replication - Allows preparation of single-stranded DNA.
