pHrodo™ Red Zymosan Bioparticles™ Conjugate for Phagocytosis
New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.
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Good product but needs tweaking and calibrations.
Macrophage phagocytosis
The instruction booklet has many options and it is hard to discern the correct one. Some information is missing like the frequency to sonicate the product. There is no positive control. In our hands, it didn't work. After-sale service was good and it was determined to be a lot issue.
Review Date: 24 Jun 2022 | Thermo Fisher Scientific
- Specific detection of phagocytosis and endocytosis
- Reduced signal variability and improved timing in sensitive experiments
- Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo™ Red dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.
Use the ready-made pHrodo™ Red Zymosan BioParticles™ Conjugate in imaging or flow applications, or pHrodo™ Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
In addition, pHrodo™ dye is also available in green color, with E. coli, zymosan, S. aureus, and dextran 10,000 conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
