Pierce™ Coomassie Plus (Bradford) Assay Kit
The Thermo Scientific Pierce Coomassie Plus Protein Assay is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent to quickly measure (A595nm) total protein concentration compared to a protein standard. The Pierce Coomassie Plus Assay Reagent is a single, ready-to-use solution for measuring protein concentration. Simply add the reagent to equal volumes of samples and standards, mix and then measure the…
Easy to use assay
Determination of protein concentration
Bradford assay kits are extremely easy to use and may find applications ranging from undergraduate teaching to research labs.
Review Date: 9 Mar 2017
The Thermo Scientific Pierce Coomassie Plus Protein Assay is a ready-to-use, reducing agent-compatible, improved Bradford assay reagent to quickly measure (A595nm) total protein concentration compared to a protein standard.
The Pierce Coomassie Plus Assay Reagent is a single, ready-to-use solution for measuring protein concentration. Simply add the reagent to equal volumes of samples and standards, mix and then measure the absorbance at 595nm. The assay costs only pennies per sample and can be performed in either test tube or microplate format. The Pierce Coomassie Plus Assay Reagent provides increased linearity of response and only half the protein-to-protein variability of other commercial Bradford assay formulations.
Features of the Coomassie Plus Protein Assay:
- Colorimetric—measure with a standard spectrophotometer or plate reader (595nm)
- Easy to use—single reagent; no working reagent preparation required
- Fast—almost immediate color development; add, mix and read results
- Broad range—detects protein concentration in the range 1 to 1500 µg/mL
- Better—improved linearity and response-uniformity compared to traditional Bradford formulations
- Flexible—microplate and cuvette protocols provided with the instructions and adaptable to several target working ranges
